Reputation: 6314
Trying to produce a gene-expression heatmap using R
's plotly
. The gene names are quite long and the dimensions are quite big:
require(permute)
require(plotly)
set.seed(1)
mat <- matrix(shuffle(c(rnorm(5000,2,1),rnorm(5000,-2,1))),nrow=2500,ncol=4)
rownames(mat) <- paste("very_long_gene_name",1:2500,sep=".")
colnames(mat) <- paste("s",1:4,sep=".")
Clustering:
hc.col <- hclust(dist(t(mat)))
dd.col <- as.dendrogram(hc.col)
col.order <- order.dendrogram(dd.col)
hc.row <- hclust(dist(mat))
dd.row <- as.dendrogram(hc.row)
row.order <- order.dendrogram(dd.row)
mat <- mat[row.order,col.order]
Producing the plot and saving the html file:
heatmap.plotly <- plot_ly(x=colnames(mat),y=rownames(mat),z=mat,type="heatmap",colors=colorRamp(c("darkblue","white","darkred")))
htmlwidgets::saveWidget(heatmap.plotly,"heatmap.plotly.html")
The figure I get has the gene names cut off, and I'm not sure it's presenting all the data:
Any idea how to fix these two issues?
Upvotes: 1
Views: 1149
Reputation: 3369
Increase your margins
m <- list(
l = 200,
r = 10,
b = 50,
t = 10,
pad = 2
)
heatmap.plotly <- plot_ly(x=colnames(mat),y=rownames(mat),z=mat,type="heatmap",
colors=colorRamp(c("darkblue","white","darkred"))) %>%
layout(margin = m)
heatmap.plotly
Upvotes: 3