Reputation: 6314
I have an experimental design to which I'd like to fit a linear regression model.
Here's the design data.frame
:
design.df <- data.frame(batch=rep(c(1:3,1:3),4),
species=rep(c(rep("mouse",3),rep("rat",3)),4),
sex=rep(c(rep("M",12),rep("F",12))),
stringsAsFactors = F)
design.df$species
and design.df$sex
are both factors
:
design.df$species <- factor(design.df$species,levels=c("mouse","rat"))
design.df$sex <- factor(design.df$sex,levels=c("F","M"))
The contrast encoding of design.df$species
should be contr.treatment
whereas that of design.df$sex
should be contr.sum
.
To set it it as a model.matrix
I thought perhaps this could work:
contrasts.list <- list(batch=NA,species="contr.treatment",sex="contr.sum")
design.mat <- model.matrix(as.formula(paste0("~",paste(model.factors,collapse="+"))),contrasts=contrasts.list,data=design.df)
Obviously it doesn't work according to the error I get:
Error in `contrasts<-`(`*tmp*`, value = contrasts.arg[[nn]]) :
contrasts apply only to factors
So my question is how do I get the model.matrix
from design.df
according to the contrasts.list
I specify?
Upvotes: 1
Views: 1093
Reputation: 206546
You are using a variable model.factors
that's not defined anywhere. Not sure what the goal is. If you just wanted all these values as covariates, you can do
contrasts.list <- list(species="contr.treatment", sex="contr.sum")
design.mat <- model.matrix(~., contrasts=contrasts.list, data=design.df)
Note that your contrasts.list
should only have values for the factor variables. Do not include batch
.
Upvotes: 1