CODEWITHSUNDEEP
windowsblastncbi
Ralph
Ralph

Reputation: 1

i get an error with magicblast.exe from NCBI in windows without further information what could be wrong

i tried to use the ncbi blast downloadable database from their ftp https://ftp.ncbi.nlm.nih.gov/blast/db/ with the magicblast.exe for winx64, extracted the tar.gz archive like stated in the readme to a folder called virus and used the following command to query my FAST genetic data for viruses in windows x64 from the command line with administrator rights:

magicblast.exe -db D:\BLAST\bin\virus\ref_viruses_rep_genomes -query F:\Genetics\XXXXX_1.fq.gz -outfmt tabular -out result.csv

but i got this error:

  1. Warning: (210.70) [magicblast] [CZipDecompressor::Process] buffer error; error code = -5, number of processed bytes = 1554

  2. BLAST query/options error: FASTA parse error: defline expected

for the second error i found a solution, i use a fastq file and not a fasta file, therefore i had to add -infmt fastq, then this error was gone, but the first error still exists.

i don't know what could be wrong here..

it looks like an Unarchive error from the magicblast.exe, but i googled for this error and didn't find any information related to these errors.

Upvotes: 0

Views: 42

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